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Secondary cultures of embryonic rat fibroblasts [NEUPERT et al., Exp. 7, 19-28 (1972)] were cultured in 21% and 5% O2 concentration at pH 6.6 and 7.4 for 6 or 8 days. The acid mucopolysaccharides were isolated and fractionated by alcohol precipitation, papain digestion, CPC-precipitation and fractionation after the microtechniques of SVEJCAR and ROBERTSON [see KITTLICK and NEUPERT, Exp. 7, 7-18 (1972)].
Cells and medium were investigated together. The results were related to cell density and compared with the glucose and lactate values [see KITTLICK and NEUPERT, Exp. 10, 109-114 (1975)]. Concerning the question of interrelations of MPS-synthesis and glycolysis a survey on literature is given. Our own test results were as follows: 1.
Hypoxia (5% O2) did not influence MPS-total synthesis. Depending on cell density the individual MPS fractions were different in their reaction to hypoxia. Hyaluronic acid (and heparan sulphate) in the MPS-pattern showed other behaviour than chondroitin sulphate and dermatan sulphate, respectively. At low cell density hypoxia effectuated increase in hyaluronic acid and decrease in chondroitin sulphate and dermatan sulphate, respectively. At high cell density hypoxia effectuated decrease in hyaluronic acid and increase in chondroitin sulphate and dermatan sulphate, respectively. Possible relationship to processes in the tissue is discussed.
Adult Children of Alcoholic/Dysfunctional Families. Meetings held 7 p.m., Tuesdays, at Santa Clara Church of Christ, 175 Santa Clara Ave. 541-729-9019, 541-431-0296. Meetings held See alanonlaneor.us for meeting schedule. Alcoholics Anonymous Meetings held See aaevi.org for meeting schedule.
ALS Lou Gehrig’s Disease support group. Meetings held 3 p.m., second Wednesday, at Hilyard Community Center, 2580 Hilyard St. Alzheimer’s support group Meetings held 1 p.m., first and third Mondays, at United Lutheran Church, 2230 Washington St. Asperger Syndrome Family Support Support for family and friends of those with Asperger Syndrome. Meetings held 6 p.m. Wednesdays beginning March 20, at 105 West Q St., Springfield 541-221-0900.
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